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pak3 thr421 cell signaling 2601  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pak3 thr421 cell signaling 2601
    Pak3 Thr421 Cell Signaling 2601, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+pak3+thr421/Phospho-PAK1+(Thr423)%2FPAK2+(Thr402)+Antibody/pmc06186433-154-38-40
    Average 95 stars, based on 260 article reviews
    pak3 thr421 cell signaling 2601 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: Generation and Characterization of Conditional Heparin-Binding EGF-Like Growth Factor Knockout Mice
    Article Snippet: .. Samples containing equivalent amounts of protein were separated on 10% sodium dodecyl sulfatepolyacrylamide gels and transferred for 2 hr at 70 V to a polyvinylidene difluoride membrane using the method of Towbin et al . Membranes were then incubated for 1 hr in blocking solution containing 4% dry milk in T-TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 0.1% Tween 20) at room temperature and then incubated overnight at 4°C with antibodies against PSD-95 (1∶250, Transduction Laboratories, Lexington, KY, USA), NR1, NR2A (1∶1000, Upstate Biotechnology, Lake Placid, NY, USA), Nr2B (R&D Systems, Minneapolis, MN, USA), EGF receptor, ErbB4 (1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-CaMKII (p-Thr286/p-Thr287) (1∶5000), anti-CaMKII (1∶5000),β-tubulin (1∶5000) ERK, p-ERK, AKT, p-AKT, PLCγ, p-PLCγ, phospho-PAK1 (Thr423), phospho-PAK2 (Thr402), or phospho-PAK3 (Thr421) (1∶1000, Cell Signaling Technology, Beverly, MA, USA) in blocking solution. .. After washing in T-TBS, membranes were incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG to visualize primary antibodies using enhanced chemiluminescence plus western blotting detection reagent (Amersham Pharmacia Biotech, Amersham, UK), according to the manufacturer's protocol.

    Incubation:

    Article Title: Generation and Characterization of Conditional Heparin-Binding EGF-Like Growth Factor Knockout Mice
    Article Snippet: .. Samples containing equivalent amounts of protein were separated on 10% sodium dodecyl sulfatepolyacrylamide gels and transferred for 2 hr at 70 V to a polyvinylidene difluoride membrane using the method of Towbin et al . Membranes were then incubated for 1 hr in blocking solution containing 4% dry milk in T-TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 0.1% Tween 20) at room temperature and then incubated overnight at 4°C with antibodies against PSD-95 (1∶250, Transduction Laboratories, Lexington, KY, USA), NR1, NR2A (1∶1000, Upstate Biotechnology, Lake Placid, NY, USA), Nr2B (R&D Systems, Minneapolis, MN, USA), EGF receptor, ErbB4 (1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-CaMKII (p-Thr286/p-Thr287) (1∶5000), anti-CaMKII (1∶5000),β-tubulin (1∶5000) ERK, p-ERK, AKT, p-AKT, PLCγ, p-PLCγ, phospho-PAK1 (Thr423), phospho-PAK2 (Thr402), or phospho-PAK3 (Thr421) (1∶1000, Cell Signaling Technology, Beverly, MA, USA) in blocking solution. .. After washing in T-TBS, membranes were incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG to visualize primary antibodies using enhanced chemiluminescence plus western blotting detection reagent (Amersham Pharmacia Biotech, Amersham, UK), according to the manufacturer's protocol.

    Blocking Assay:

    Article Title: Generation and Characterization of Conditional Heparin-Binding EGF-Like Growth Factor Knockout Mice
    Article Snippet: .. Samples containing equivalent amounts of protein were separated on 10% sodium dodecyl sulfatepolyacrylamide gels and transferred for 2 hr at 70 V to a polyvinylidene difluoride membrane using the method of Towbin et al . Membranes were then incubated for 1 hr in blocking solution containing 4% dry milk in T-TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 0.1% Tween 20) at room temperature and then incubated overnight at 4°C with antibodies against PSD-95 (1∶250, Transduction Laboratories, Lexington, KY, USA), NR1, NR2A (1∶1000, Upstate Biotechnology, Lake Placid, NY, USA), Nr2B (R&D Systems, Minneapolis, MN, USA), EGF receptor, ErbB4 (1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-CaMKII (p-Thr286/p-Thr287) (1∶5000), anti-CaMKII (1∶5000),β-tubulin (1∶5000) ERK, p-ERK, AKT, p-AKT, PLCγ, p-PLCγ, phospho-PAK1 (Thr423), phospho-PAK2 (Thr402), or phospho-PAK3 (Thr421) (1∶1000, Cell Signaling Technology, Beverly, MA, USA) in blocking solution. .. After washing in T-TBS, membranes were incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG to visualize primary antibodies using enhanced chemiluminescence plus western blotting detection reagent (Amersham Pharmacia Biotech, Amersham, UK), according to the manufacturer's protocol.

    Transduction:

    Article Title: Generation and Characterization of Conditional Heparin-Binding EGF-Like Growth Factor Knockout Mice
    Article Snippet: .. Samples containing equivalent amounts of protein were separated on 10% sodium dodecyl sulfatepolyacrylamide gels and transferred for 2 hr at 70 V to a polyvinylidene difluoride membrane using the method of Towbin et al . Membranes were then incubated for 1 hr in blocking solution containing 4% dry milk in T-TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 0.1% Tween 20) at room temperature and then incubated overnight at 4°C with antibodies against PSD-95 (1∶250, Transduction Laboratories, Lexington, KY, USA), NR1, NR2A (1∶1000, Upstate Biotechnology, Lake Placid, NY, USA), Nr2B (R&D Systems, Minneapolis, MN, USA), EGF receptor, ErbB4 (1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-CaMKII (p-Thr286/p-Thr287) (1∶5000), anti-CaMKII (1∶5000),β-tubulin (1∶5000) ERK, p-ERK, AKT, p-AKT, PLCγ, p-PLCγ, phospho-PAK1 (Thr423), phospho-PAK2 (Thr402), or phospho-PAK3 (Thr421) (1∶1000, Cell Signaling Technology, Beverly, MA, USA) in blocking solution. .. After washing in T-TBS, membranes were incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG to visualize primary antibodies using enhanced chemiluminescence plus western blotting detection reagent (Amersham Pharmacia Biotech, Amersham, UK), according to the manufacturer's protocol.



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